Antimicrobial compounds

A compound for use as an antimicrobial having a formula (A).

1. A method of treating a bacterial infection in a subject comprising administering to the subject an effective amount of a compound or pharmaceutically acceptable salt thereof, the compound having a structure

wherein

each of W₁, W₂, W₃, and W₄is the same and is C2-4 alkyl, C2-4 substituted alkyl, or C2 alkene;

each of Z₁, Z₂, Z₃and Z₄is the same and is

each of R₁, R₂, R₃, R₄, and R₅is independently H or C_1-8heteroalkyl, and the C_1-8heteroalkyl comprises CO₂H or ester thereof, with the proviso that at least one of R₁, R₂, R₃, R₄, and R₅is C_1-8heteroalkyl.

2. The method of claim 1, wherein the bacterial infection results from a Gram-positive bacteria.

3. The method of claim 2, wherein the bacteria is a methicillin-resistant Staphylococcus aureus (MRSA) or Clostridium difficile.

4. The method of claim 1, wherein the compound is:

5. The method of claim 4, wherein the bacterial infection results from a Gram-positive bacteria.

6. The method of claim 5, wherein the bacteria is a methicillin-resistant Staphylococcus aureus (MRSA) or Clostridium difficile.

7. The method of claim 1, wherein the compound is

8. The method of claim 1, wherein the compound is selected from the group consisting of

The present invention relates to novel aryl compounds. More particularly the present invention relates to novel aryl compounds and their use as antimicrobials to treat bacterial infections or diseases.

Compounds with antimicrobial properties have attracted great interest in recent times as a result of an increase in the prevalence of infections caused by Gram-positive bacteria, resulting in serious or fatal diseases. Furthermore, the regular use of broad spectrum antibiotic formulas has led to the increased occurrence of bacterial strains resistant to some antimicrobial formulations.

Novel antimicrobial compounds have the potential to be highly effective against these types of treatment-resistant bacteria. The pathogens, having not previously been exposed to the antimicrobial formulation, may have little to no resistance to the treatment.

General

Those skilled in the art will appreciate that the invention described herein is susceptible to variations and modifications other than those specifically described. It is to be understood that the invention includes all such variation and modifications. The invention also includes all of the steps, features, compositions and compounds referred to or indicated in the specification, individually or collectively and any and all combinations or any two or more of the steps or features.

The present invention is not to be limited in scope by the specific embodiments described herein, which are intended for the purpose of exemplification only. Functionally equivalent products, compositions and methods are clearly within the scope of the invention as described herein.

The entire disclosures of all publications (including patents, patent applications, journal articles, laboratory manuals, books, or other documents) cited herein are hereby incorporated by reference, which means that it should be read and considered by the reader as part of this text. That the document, reference, patent application or patent cited in this text is not repeated in this text is merely for reasons of conciseness. No admission is made that any of the references constitute prior art or are part of the common general knowledge of those working in the field to which this invention relates.

Throughout this specification, unless the context requires otherwise, the term antimicrobial is understood to include compounds with antibacterial properties.

Throughout this specification, unless the context requires otherwise, the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.

Other definitions for selected terms used herein may be found within the detailed description of the invention and apply throughout. Unless otherwise defined, all other scientific and technical terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which the invention belongs.

Use of Compounds

In one form, the present invention provides for the use of a compound of Formula A, or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the therapeutic treatment of bacterial infection or disease in a subject in need thereof.

Where;

• • W is a linking unit and Z is a peripheral unit. V, linking unit W and peripheral unit Z are all linked by co-valent bonds;
  • n=1, 2, 3, or 4.
  • V=a six membered aromatic ring.
  • The, or each W may be independently selected from:
    • i. C_2-4alkyl groups;
    • ii. C_2-4substituted alkyl groups;
    • iii. C₂E-alkene, with V and Z in the 1 and 2 positions;
    • iv. C₂Z-alkene, with V and Z in the 1 and 2 positions;
    • v. C₂-alkyne;
  • The, or each Z may be selected from any one or more of:

• • R_nmay be independently selected from any one or more of i-xxxvii, with at least one R group being independently selected from any one of ii-xxxvii:
    • i. —H,
    • ii. -Halo,
    • iii. C_1-8alkyl,
    • iv. C_1-8heteroalkyl,
      • a) Carboxylic acids and related derivatives independently selected from:
      • b) Carboxylic acid,

• • • • c) Thiocarboxylic acid,

• • • • d) Esters,

• • • • e) Thioester,

• • • • f) Dithioester,

• • • v. Amide derivatives of carboxylic acids independently selected from:
      • a) Amide,

• • • • b) Thioamide,

• • • vi. Aldehydes, ketones and their derivatives independently selected from:
      • a) Aldehyde,

• • • • b) Thial,

• • • • c) Ketones,

• • • • d) Thioketones,

• • • • e) Acetals,

• • • • f) Dithioacetals,

• • • vii. Amines, alkyl amines and their derivatives independently selected from:
      • a) Amines,

• • • • b) Amides,

• • • • c) Thioamide,

• • • • d) Ammonium salts,

• • • • e) Alkyl amines,

• • • •  where n=1-3
      • f) Alkyl amides,

• • • •  where n=1-3
      • g) Alkyl thioamides,

• • • •  where n=1-3
      • h) Alkyl ammonium salts,

• • • •  where n=1-3,
      • i) Imines,

• • • • j) Guanidines,

• • • • k) Amidine,

• • • viii. Nitrile (cyano), —C≡N,
    • ix. Isonitrile, —N⁺≡C⁻,
    • x. Cyanate, —O—C≡N,
    • xi. Isocyanate, —N═C═O,
    • xii. Thiocyanate, —S—C≡N,
    • xiii. Isothiocyanate, —N═C═S,
    • xiv. Azo, —N═NH
    • xv. Nitro,

• • • xvi. Nitrite, —O—N═O,
    • xvii. Nitriso, —N═O,
    • xviii. N-terminal peptide sequences,

• • •  where x=1-3 and R^pepis any group resulting in the formation of an amino acid.
    • xix. C-terminal peptide sequences,

• • •  where x=1-3 and R^pepis any group resulting in the formation of an amino acid.
    • xx. Phosphorus based substituents, where the phosphorus atom is in either the 3+ or 5+ oxidation state, independently selected from:
      • a) Phosphines,

• • • • b) Phosphine oxides,

• • • • c) Phosphites,

• • • • d) Phosphates,

• • • • e) Phosphinites,

• • • • f) Phosphinates,

• • • • g) Phosphinites,

• • • • h) Phosphonates,

• • • xxi. sulfur based substituents,
      • a) Sulfate,

• • • • b) Sulfone,

• • • • c) Sulfoxide,

• • • • d) Sulfinic acids,

• • • • e) Sulfimines,

• • • • f) Sulfonamides,

• • • xxii. Boron based substituents,
      • a) Boronic acids,

• • • • b) Boronic esters,

• • • xxiii. Semicarbazones,

• • • xxiv. Thiosemicarbazones,

• • • xxv. Cyanimide,

• • • xxvi. Hydrazone,

• • • xxvii. Oxime,

• • • xxviii. Nitroamine,

• • • xxix. Nitronate,

• • • xxx. Nitrone,

• • • xxxi. Carbonates,

• • • xxxii. Carbamates,

• • • xxxiii. Dithiocarbamates,

• • • xxxiv. A 5- or 6-membered saturated heterocyclic ring containing 1 to 3 heteroatoms chosen independently from nitrogen, sulfur and oxygen.
    • xxxv. A 5- or 6-membered saturated heteroaromatic ring containing 1 to 5 heteroatoms chosen independently from nitrogen, sulfur and oxygen.
    • xxxvi. A 5-membered unsaturated heterocyclic ring containing, one or two double bonds and containing 1 to 5 heteroatoms chosen independently from nitrogen, sulphur and oxygen.
    • xxxvii. A 6 membered ring containing 1 to 3 double bonds and containing 1 to 5 heteroatoms chosen independently from nitrogen, sulfur and oxygen dependant on ring size.

The present invention further provides for the use of a compound of Formula B:

or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the therapeutic treatment of bacterial infection or disease in a subject in need thereof, wherein:

• • n′=2, 3 or 4
  • R′ comprises any one of C₂-alkyl, C₂-alkene or C₂-alkyne; and
  • R″ is independently selected from NH₂, CH₃, OH, —COCH₃, —OC(CH₃)₃, OCH₃, OCH₂CH₃, OCH(CH₃)₂, —CONH₂, COOH, COOCH₃, or COOCH₂CH₃

In a preferred form, the present invention provides for the use of a compound of Formula B:

or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the therapeutic treatment of bacterial infection or disease in a subject, in need thereof wherein:

• • n′=2, 3 or 4
  • R′=C₂-alkyl or C₂-alkene
  • R″ is independently selected from COOCH₃, COOCH₂CH₃or COOH

In a further preferred form, the invention provides for the use of a compound of Formula C:

or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the therapeutic treatment of bacterial infection or disease in a subject in need thereof.

The compound of Formula C is also known as NAL135B, 135B and TSB007 (see FIG. 7).

In an alternative aspect, the compound provided for the use of a compound selected from the following list of analogues of formula C: TSB049, TSB063, TSB037, TSB041, TSB019, TSB023, TSB025, TSB065, TCT003, TSB001, TSB009, TSB053, TAB001, TCB001, TCB003 (see FIGS. 8 to 12 for structure information). More preferably, the compound is TSB065 or TCB001.

The use of a compound of any one of Formulas A, B or C, or analogues thereof, or pharmaceutically acceptable salts thereof in the manufacture of a medicament for the therapeutic treatment of bacterial infection or disease in a subject in need thereof, wherein the bacterial infection or disease results from Gram-positive bacteria.

Method of Treatment

The compounds of the present invention have antimicrobial activity, and thus are useful for treatment of bacterial infections. Thus, the present invention also provides a method of treating a bacterial infection in a subject comprising the step of administering to the subject an effective amount of a compound of Formula A and variants described herein.

The present invention further provides a method of treating a bacterial infection in a subject comprising the step of administering to the subject an effective amount of a compound of Formula B:

Wherein:
n′=2, 3 or 4
R′ comprises any one of C₂-alkyl, C₂-alkene or C₂-alkyne; and
R″ is independently selected from NH₂, CH₃, OH, —COCH₃, —OC(CH₃)₃, OCH₃, OCH₂CH₃, OCH(CH₃)₂, —CONH₂, COOH, COOCH₃, or COOCH₂CH₃.

In a preferred form the present invention provides a method of treating a bacterial infection in a subject comprising the step of administering to the subject an effective amount of a compound of Formula B wherein:

n′=2, 3 or 4
R′=c₂-alkyl or C₂-alkene
R″ is independently selected from COOCH₃, COOCH₂CH₃or COOH

In a further preferred form, the present invention provides a method of treating a bacterial infection in a subject comprising the step of administering to the subject an effective amount of a compound of Formula C:

In an alternative aspect, the present invention provides a method of treating a bacterial infection in a subject comprising the step of administering to the subject an effective amount of a compound selected from the following list of analogues of formula C: TSB049, TSB063, TSB037, TSB041, TSB019, TSB023, TSB025, TSB065, TCT003, TSB001, TSB009, TSB053, TAB001, TCB001, TCB003 (see FIGS. 8 to 12 for structure information). More preferably, the compound is TSB065 or TCB001.

The compounds of the present invention are understood to be effective against both Gram positive bacteria and Gram negative bacteria.

Examples of Gram-negative bacteria which the compounds of the present invention may preferably be used against include: Aeromonas hydrophila, Citrobacter freundii, Escherichia coli, Klebsiella edwardsii, Proteus mirabilis, Salmonella enterica subsp. enterica serovar Typhimurium, Moraxella catarrhalis, Shigella flexneri, Stenotrophomonas maltophilia, Vibrio cholerae (non-toxigenic) and Yersinia enterocolitica.

The compounds of the present invention are understood to be most effective against Gram-positive bacteria. Examples of Gram-positive bacteria which the compounds of the present invention may preferably be used against include: Bacillus cereus, Bacillus subtilise, Clostridium difficile, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Staphylococcus aureus (methicillin resistant), Staphylococcus epidermidis, Staphylococcus xylosus, Streptococcus pneumonia, and Streptococcus pyogenes.

The compounds of the present invention are understood to be particularly effective against methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile.

Compositions

The compounds of the present invention may be formulated into compositions for administration.

Thus, the present invention also provides a composition comprising a therapeutically-effective amount of a compound of Formula A and variants described herein, and a pharmaceutically acceptable carrier or diluent.

The present invention also provides a composition comprising a therapeutically-effective amount of a compound having a Formula B and a pharmaceutically acceptable carrier or diluents:

n=2, 3 or 4
R′ comprises any one of C₂-alkyl, C₂-alkene or C₂-alkyne; and
R″ is independently selected from NH₂, CH₃, OH, —COCH₃, —OC(CH₃)₃, OCH₃, OCH₂CH₃, OCH(CH₃)₂, —CONH₂, COOH, COOCH₃, or COOCH₂CH₃.

In a preferred form, the present invention provides a composition comprising a therapeutically-effective amount of a compound having a Formula B and a pharmaceutically acceptable carrier or diluents

wherein:
n=2, 3 or 4
R′=c₂-alkyl or C₂-alkene
R″ is independently selected from COOCH₃, COOCH₂CH₃or COOH.

The present invention also provides a composition comprising a therapeutically-effective amount of a compound having a Formula C and a pharmaceutically acceptable carrier or diluent.

In an alternative aspect of the present invention, there is provided a composition comprising a therapeutically-effective amount of a compound selected from the following list of analogues of compound C: TSB049, TSB063, TSB037, TSB041, TSB019, TSB023, TSB025, TSB065, TCT003, TSB001, TSB009, TSB053, TAB001, TCB001, TCB003 (see FIGS. 8 to 12 for structure information) and a pharmaceutically acceptable carrier or diluent. More preferably, the compound is TSB065 or TCB001.

With respect to use, methods of treatment and compositions comprising the compounds having a Formula A, B or C, or analogues thereof and variants described herein:—

V is preferably, a phenyl or a pyridal ring.

Where V is a phenyl ring and:

• • i. n=3, the W—Z group may be substituted at positions 1, 2, and 3; or 1, 2, and 4; or 1, 3 and 5, with A¹, A²and A³occupying remaining positions on the ring:

• • ii. n=4, W—Z may be substituted at positions 1, 2, 3 and 4; 1, 2, 3 and 5; 1, 2, 4 and 5 with A¹and A²occupying remaining positions on the ring:

• • iii. n=5, W—Z may be substituted at positions 1, 2, 3, 4 and 5 with A¹occupying the remaining position on the ring:

• • iv. n=6, W—Z is substituted into each of positions 1, 2, 3, 4, 5 and 6.

Where V is a pyridal ring, and:

• • i. n=3, W—Z may be substituted into positions 2, 3, and 4; or 2, 3, and 5; or 2, 3, and 6; or 2, 5, and 6; or 2, 4 and 6; or 3, 4, and 5, with A¹and A²occupying the remaining positions on the pyridal ring, and where the pyridal nitrogen (N) occupies position 1.

• • ii. n=4, W—Z may be substituted into positions, 2, 3, 4, and 5; or 2, 3, 5, and 6, with A¹occupying the remaining position on the pyridal ring, and where the pyridal nitrogen (N) occupies position 1.

• • iii. n=5, W—Z may be substituted into positions, 2, 3, 4, 5 and 6; and where the pyridal nitrogen (N) occupies position 1.

Where the, or each W is in the form of a C₂E-alkene, with V and Z in positions 1 and 2, positions 3 and 4 are each preferably independently substituted with a hydrogen or C_1-4alkyl.

Where the, or each W is in the form of a C₂Z-alkene, with V and Z in positions 1 and 2, positions 3 and 4 are each preferably independently substituted with a hydrogen or C_1-4alkyl.

Where R_nis a carboxylic acid; the carboxylic acid is preferably in the form of —COOR^a.

More preferably, R^ais any one of hydrogen, alkyl (C_1-4).

Where R_nis an amide derivative of a carboxylic acid, R^aand R^bare independently selected from hydrogen or alkyl (C_1-4).

Where R_nis an aldehyde, ketone, or a derivative thereof, R^aand R^bare independently selected from hydrogen or alkyl (C_1-4).

Where R_nis an amine, alkyl amine or a derivative thereof, R^a, R^b, and R^care independently selected from hydrogen or alkyl (C_1-4).

Where R_nis a saturated 5 or 6 membered heterocyclic ring, the heterocycle is preferably any one of piperidinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isooxazolidinyl, pyrrolidinyl, imidazolidinyl, piperazinyl, tetrahydrofuranyl, dioxaanyl, dithanyl, and pyrazolidinyl.

The saturated heterocyclic ring may be unsubstituted. Alternatively, the saturated heterocyclic ring may be substituted with 1 to 3 substituents independently selected from:

• • 1. Halogen, independently selected from Br, I, Cl, F
  • 2. Alkyl, independently selected from methyl, ethyl, propyl or butyl

Where R_nis a 5 or 6 membered heteroaromatic ring, the heteroaromatic is preferably any one of thienyl, pyridyl, imidazolyl, pyrrolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isooxazolyl, pyrazinyl, pyrimidinyl, triazolyl, tetrazolyl, furanyl, triazinyl, and pyridazinyl.

The heteroaromatic ring may be unsubstituted. Alternatively, the heteroaromatic ring may be substituted with 1 to 5 substituents independently selected from:

• • 1. Halogen, independently selected from Br, I, Cl, F
  • 2. Alkyl, independently selected from methyl, ethyl, propyl or butyl

Where R_nis an unsaturated 5 or 6 membered heterocyclic ring, the heterocycle is preferably any one of oxolanyl, imidazolinyl, pyrazolinyl, thiazolidinyl, oxazolininyl, dithiolanyl, and dioxolanyl.

More preferably, the unsaturated heterocyclic ring is not an aromatic.

The unsaturated heterocyclic ring may be unsubstituted. Alternatively, the unsaturated heterocyclic ring may be substituted with 1 to 5 substitutents independently selected from:

• • 1. Halogen, independently selected from Br, I, Cl, F
  • 2. Alkyl, independently selected from methyl, ethyl, propyl or butyl.

R′ is preferably in the form of any one of C₂-alkyl or C₂-alkene, and R″ is independently selected from COOCH₃, COOCH₂CH₃or COOH.

Preferably, where n′=2, R′—C₆H₄—R″, is substituted at positions 1 and 3.

Preferably, where n′=3, R′—C₆H₄—R″, is substituted at positions 1, 3 and 5; or 1, 2 and 4.

Preferably, where n′=3, R′—C₆H₄—R″, is substituted at positions 1, 2, 4 and 5.

R^peppreferably has a peptide comprised of one or more α-amino acids, independently selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine or valine.

The present invention provides use of a compound of Formulas A, B or C, or analogues thereof, or pharmaceutically acceptable salts thereof in the manufacture of a medicament for the therapeutic treatment of bacterial infection or disease in a subject in need thereof, wherein the bacterial infection or disease results from Gram-positive bacteria.

The precise composition of the present invention will vary according to a wide range of commercial and scientific criteria. Methods for the preparation of pharmaceutical compositions comprising one or more active ingredients are generally known in the art. Such compositions will generally be formulated for the mode of delivery that is to be used and will usually include one or more pharmaceutically acceptable carriers.

Generally, examples of suitable carriers, excipient and diluents include, without limitation, water, saline, ethanol, dextrose, glycerol, lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphates, alginate, tragacanth, gelatine, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water syrup, methyl cellulose, methyl and propylhydroxybenzoates, talc magnesium stearate and mineral oil or combinations thereof. The formulations can additionally include lubricating agents, pH buffering agents, wetting agents, emulsifying and suspending agents, preserving agents, sweetening agents or flavouring agents.

(a) Topicals

The pharmaceutical composition may be adapted for topical application. In this regard, various topical delivery systems may be appropriate for administering the compositions of the present invention depending up on the preferred treatment regimen. Topical formulations may be produced by dissolving or combining the compound of the present invention in an aqueous or nonaqueous carrier. In general, any liquid, cream, or gel or similar substance that does not appreciably react with the compound or any other of the active ingredients that may be introduced into the composition and which is non-irritating is suitable. Appropriate non-sprayable viscous, semi-solid or solid forms can also be employed that include a carrier compatible with topical application and have dynamic viscosity preferably greater than water.

Suitable formulations are well known to those skilled in the art and include, but are not limited to, solutions, suspensions, emulsions, creams, gels, ointments, powders, liniments, salves, aerosols, transdermal patches, etc, which are, if desired, sterilised or mixed with auxiliary agents, e.g. preservatives, stabilisers, emulsifiers, wetting agents, fragrances, colouring agents, odour controllers, thickeners such as natural gums, etc. Particularly preferred topical formulations include ointments, creams or gels.

Ointments generally are prepared using either (1) an oleaginous base, i.e., one consisting of fixed oils or hydrocarbons, such as white petroleum or mineral oil, or (2) an absorbent base, i.e., one consisting of an anhydrous substance or substances which can absorb water, for example anhydrous lanolin. Customarily, following formation of the base, whether oleaginous or absorbent, the active ingredient is added to an amount affording the desired concentration.

Creams are oil/water emulsions. They consist of an oil phase (internal phase), comprising typically fixed oils, hydrocarbons and the like, waxes, petroleum, mineral oil and the like and an aqueous phase (continuous phase), comprising water and any water-soluble substances, such as added salts. The two phases are stabilised by use of an emulsifying agent, for example, a surface active agent, such as sodium lauryl sulfite; hydrophilic colloids, such as acacia colloidal clays, veegum and the like. Upon formation of the emulsion, the compound can be added in an amount to achieve the desired concentration.

Gels comprise a base selected from an oleaginous base, water, or an emulsion-suspension base. To the base is added a gelling agent that forms a matrix in the base, increasing its viscosity. Examples of gelling agents are hydroxypropyl cellulose, acrylic acid polymers and the like. Customarily, the compound is added to the formulation at the desired concentration at a point preceding addition of the gelling agent.

The amount of compound incorporated into a topical formulation is not critical; the concentration should be within a range sufficient to permit ready application of the formulation such that an effective amount of the compound is delivered.

(b) Oral Formulations

The pharmaceutical composition may be adapted for oral delivery. In this regard, the compound can be administered as an oral preparation adapted in such a manner that facilitates delivery of a therapeutically effective concentration of the compound.

The effective dosages of the compound, when administered orally, must take into consideration the diluent, preferably water. The composition preferably, contains 0.05% to about 100% by weight active ingredient and more preferably about 10% to about 80% by weight. When the compositions are ingested, desirably they are taken on an empty stomach.

Contemplated for use herein are oral solid dosage forms including tablets, capsules, pills, troches or lozenges, cachets or pellets. Also, liposomal or proteinoid encapsulation may be used to formulate the present compositions. Liposomal encapsulation may be used and the liposomes may be derivatised with various polymers. In general, the formulation will include the compound and inert ingredients that allow for protection against the stomach environment and release of the biologically active material in the intestine.

The location of release may be the stomach, the small intestine (the duodenum, the jejunem, or the ileum), or the large intestine. One skilled in the art has available formulations that will not dissolve in the stomach, yet will release the material in the duodenum or elsewhere in the intestine. Preferably, the release will avoid the deleterious effects of the stomach environment, either by protection of the composition or by release of the compound beyond the stomach environment, such as in the intestine.

To ensure full, gastric resistance, a coating impermeable to at least pH 5.0 may be used. Examples of the more common inert ingredients that are used as enteric coatings are cellulose acetate trimellitate (CAT), hydroxypropylmethylcellulose phthalate (HPMCP), HPMCP 50, HPMCP 55, polyvinyl acetate phthalate (PVAP), Eudragit L30D, Aquateric, cellulose acetate phthalate (CAP), Eudragit L, Eudragit S and Shellac. These coatings may be used as mixed films.

A coating or mixture of coatings that are not intended for protection against the stomach can also be used on tablets. This can include sugar coatings, or coatings that make the tablet easier to swallow. Capsules may consist of a hard shell (such as gelatine) for delivery of dry therapeutic i.e. powder; for liquid forms, a soft gelatine shell may be used. The shell material of cachets could be thick starch or other edible paper. For pills, lozenges, moulded tablets or tablet triturates, moist massing techniques can be used.

One may dilute or increase the volume of the composition with an inert material. These diluents could include carbohydrates, especially mannitol, alpha-lactose, anhydrous lactose, cellulose, sucrose, modified dextrans and starch. Certain inorganic salts may be also be used as fillers including calcium triphosphate, magnesium carbonate and sodium chloride. Some commercially available diluents are Fast-Flo, Emdex, STA-Rx 1500, Emcompress and Avicell.

Disintegrants may be included in the formulation of the compound into a solid dosage form. Materials used as disintegrants include but are not limited to starch including the commercial disintegrant based on starch, Explotab. Sodium starch glycolate, Amberlite, sodium carboxymethylcellulose, ultramylopectin, sodium alginate, gelatine, orange peel, acid carboxymethyl cellulose, natural sponge and bentonite may all be used. Another form of the disintegrants is insoluble cationic exchange resins. Powdered gums may be used as disintegrants and as binders and these can include powdered gums such as agar, Karaya or tragacanth. Alginic acid and its sodium salt are also useful as disintegrants.

Binders may be used to hold the composition together to form a hard tablet and include materials from natural products such as acacia, tragacanth, starch and gelatine. Others include methylcellulose (MC), ethyl cellulose (EC) and carboxymethyl cellulose (CMC). Polyvinyl pyrrolidone (PVP) and hydroxypropylmethyl cellulose (HPMC) could both be used in alcoholic solutions to granulate the compound.

An antifrictional agent may be included in the formulation to prevent sticking during the formulation process. Lubricants may be used as a layer between the compound and the die wall and these can include but are not limited to: stearic acid including its magnesium and calcium salts, polytetrafluoroethylene (PTFE), liquid paraffin, vegetable oils and waxes. Soluble lubricants may also be used such as sodium lauryl sulfate, magnesium lauryl sulfate, polyethylene glycol of various molecular weights and Carbowax 4000 and 6000.

Glidants that might improve the flow properties of the composition during formulation and to aid rearrangement during compression might be added. The glidants may include starch, talc, pyrogenic silica and hydrated silicoaluminate.

To aid dissolution of the compound, a surfactant might be added as a wetting agent. Surfactants may include anionic detergents such as sodium lauryl sulfate, dioctyl sodium sulfosuccinate and dioctyl sodium sulfonate. Cationic detergents might be used and could include benzalkonium chloride or benzethomium chloride. The list of potential nonionic detergents that could be included in the formulation as surfactants are lauromacrogol 400, polyoxyl 40 stearate, polyoxyethylene hydrogenated castor oil 10, 50 and 60, glycerol monostearate, polysorbate 40, 60, 65 and 80, sucrose fatty acid ester, methyl cellulose and carboxymethyl cellulose. These surfactants could be present in the formulation either alone or as a mixture in different ratios.

Controlled release formulations may be desirable. The compounds can be incorporated into an inert matrix that permits release by either diffusion or leaching mechanisms i.e., gums. Slowly degenerating matrices may also be incorporated into the formulation. Another form of a controlled release formulation is by a method based on the Oros therapeutic system (Alza Corp.), i.e. the composition is enclosed in a semipermeable membrane which allows water to enter and push the composition out through a single small opening due to osmotic effects. Some enteric coatings also have a delayed release effect.

A mix of materials might be used to provide the optimum film coating. Film coating may be carried out in a pan coater or in a fluidised bed or by compression coating.

The compound can be included in the formulation as fine multiparticulates in the form of granules or pellets of particle size about 1 mm. The formulation of the material for capsule administration could also be as a powder, lightly compressed plugs or even as tablets. The compound could be prepared by compression.

(c) Injectable Formulations

The compound can also be formulated for parenteral delivery. Pharmaceutical forms suitable for injectable use include: sterile aqueous solutions (where water-soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. Alternatively, the compounds of the invention may be encapsulated in liposomes and delivered in injectable solutions to assist their transport across cell membrane. The solution may be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol and liquid polyethylene glycol and the like), suitable mixtures thereof and vegetable oils. Proper fluidity may be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminium monostearate and gelatine.

Sterile injectable solutions may be prepared by incorporating the active compounds in the required amount in an appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilisation. Generally, dispersions are prepared by incorporating the compound into a sterile vehicle that contains the basic dispersion medium and the other ingredients. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying techniques that yield a powder of the compound plus any additional desired ingredient from previously sterile-filtered solution thereof.

Thus, the present invention also provides an injectable, stable, sterile composition comprising a compound of Formula A, or a salt thereof, in a unit dosage form in a sealed container. The compound or salt may be provided in lyophilised form capable of being reconstituted with a suitable pharmaceutically acceptable carrier to form a liquid composition suitable for injection thereof into a subject. The unit dosage form typically comprises from about 10 mg to about 10 grams of the compound or salt thereof. When the compound or salt is substantially water-insoluble, a sufficient amount of emulsifying agent which is physiologically acceptable may be employed in sufficient quantity to emulsify the compound or salt in an aqueous carrier. One such useful emulsifying agent is phosphatidyl choline.

(d) Aerosols

Pharmaceutical compositions are also provided which are suitable for administration as an aerosol, by inhalation. These compositions comprise a solution or suspension of the desired compound or a salt thereof or a plurality of solid particles of the compound or salt. The desired composition may be placed in a small chamber and nebulized. Nebulization may be accomplished by compressed air, or by ultrasonic energy to form a plurality of liquid droplets or solid particles comprising the compounds or salts.

The solid particles can be obtained by processing solid compound or a salt thereof, in any appropriate manner known in the art, such as by micronization. Commercial nebulizers are also available to provide liquid droplets of any desired size.

The liquid droplets or solid particles should have a particle size in the range of about 0.5 to about 5 microns, preferably from about 1 to about 2 microns. Most preferably, the size of the solid particles or droplets will be from about 1 to about 2 microns. Such particles or droplets may be dispensed by commercially available nebulisers or by other means known to the skilled person.

When the pharmaceutical composition suitable for administration as an aerosol is in the form of a liquid, the composition will comprise a water-soluble form of the compound or a salt thereof, in a carrier that comprises water. A surfactant may be present which lowers the surface tension of the composition sufficiently to result in the formation of droplets within the desired size range when subjected to nebulization.

In addition, the pharmaceutical composition may also include other agents. For example, preservatives, co-solvents, surfactants, oils, humectants, emollients, chelating agents, dyestuffs, stabilizers or antioxidants may be employed. Water soluble preservatives that may be employed include, but are not limited to, benzalkonium chloride, chlorobutanol, thimerosal, sodium bisulfate, phenylmercuric acetate, phenylmercuric nitrate, ethyl alcohol, methylparaben, polyvinyl alcohol, benzyl alcohol and phenylethyl alcohol. A surfactant may be Tween 80. Other suitable additives include lubricants and slip agents, such as, for example, magnesium stearate, stearic acid, talc and bentonites, substances which promote disintegration, such as starch or crosslinked polyvinylpyrrolidone, binders, such as, for example, starch, gelatin or linear polyvinylpyrrolidone, and dry binders, such as microcrystalline cellulose.

Other vehicles that may be used include, but are not limited to, polyvinyl alcohol, povidone, hydroxypropyl methyl cellulose, poloxamers, carboxymethyl cellulose, hydroxyethyl cellulose, purified, water, etc. Tonicity adjustors may be included, for example, sodium chloride, potassium chloride, mannitol, glycerin, etc. Antioxidants include, but are not limited to, sodium metabisulfite, sodium thiosulfate, acetylcysteine, butylated hydroxyanisole, butylated hydroxytoluene, etc. The indications, effective doses, compositions, contraindications, vendors etc, of the compounds in the compositions are available or are known to one skilled in the art. These agents may be present in individual amounts of from about 0.001% to about 5% by weight and preferably about 0.01% to about 2%.

Electrolytes such as, but not limited to, sodium chloride and potassium chloride may also be included in the composition.

Further, the compositions may contain microbial preservatives. Useful microbial preservatives include methylparaben, propylparaben, and benzyl alcohol. The microbial preservative is typically employed when the composition is placed in a vial designed for multidose use.

Excipients which may be used are all the physiologically acceptable solid inert substances, either inorganic or organic in nature. Inorganic substances are, for example, sodium chloride, carbonates, such as calcium carbonate, bicarbonates, aluminium oxides, silicic acids, aluminas, precipitated or colloidal silicon dioxide and phosphates. Organic substances are, for example, sugars, cellulose, foodstuffs and feedstuffs, such as milk powder, animal flours, cereal flours and shredded cereals and starches.

Finally, it will be appreciated that the compositions of the present invention may comprise a plurality of compounds as described herein.

Compounds

In one form, the invention provides a compound of Formula B:

Where:
N′=2, 3 or 4
R′ comprises any one of C₂-alkyl, C₂-alkene or C₂-alkyne; and
R″ is independently selected from NH₂, CH₃, OH, —COCH₃, —OC(CH₃)₃, OCH₃, OCH₂CH₃, OCH(CH₃)₂, —CONH₂, COOH, COOCH₃, or COOCH₂CH₃.

In a preferred form, the invention provides a compound of Formula B:

wherein
R′ comprises any one of C₂-alkyl, C₂-alkene; and
R″ is independently selected from COOH, COOCH₃, or COOCH₂CH₃.

Preferably, where n′=2, R′—C₆H₄—R″, is substituted at positions 1 and 3.

Preferably, where n′=3, R′—C₆H₄—R″, is substituted at positions 1, 3 and 5; or 1, 2 and 4.

Preferably, where n′=3, R′—C₆H₄—R″, is substituted at positions 1, 2, 4 and 5.

In accordance with a further aspect of the present invention, there is provided a compound of Formula C:

The compounds of the present invention may be provided in the form of a pharmaceutically acceptable salt.

Pharmaceutically acceptable salts for the purposes of the present invention include non-toxic cation and anion salts. Examples include, but are not limited to sodium, potassium, aluminium, calcium, lithium, magnesium, zinc and from bases such as ammonium, ethylenediamine, N-methyl-glutamine, lysine, arginine, ornithine, choline, N,N′-dibenzylethlenediamine, diethylamine, piperazine, tris(hydroxymethyl)aminomethane, tetramethylammonium, acetate, lactobionate, benzenesulfonate, laurate, benzoate, malate, bicarbonate, maleate, bisulfate, mandelate, bitratrate, meyate, borate, methylbromide, bromide, methylnitrate, calcium edetate, methylsulfate, camsylate, mucate, carbonate, napsylate, chloride, nitrate, clavulanate, N-methylglucamine, citrate, hydrochloride, oleate, edetate, oxalate, edisylate, pamoate (embonate), estolate, palmitate, esylate, pantothenate, fumarate, phosphate, diphosphate, glucepate, plygalacturonate, gluconate, salicylate, glutamate, stearate, glycollylarsanilate, sulfate, hexylresorcinate, subacetate, hydrabamine, succinate, hydrobromide, tannate, tartrate, hydroxynapthoate, teoclate, iodide, tosylate, isothionate, triethiodide, lactate, panoate and valerate.

In an alternative aspect, the compound provided by the present invention is preferably selected from the following list of analogues of compound C: TSB049, TSB063, TSB037, TSB041, TSB019, TSB023, TSB025, TSB065, TCT003, TSB001, TSB009, TSB053, TAB001, TCB001, TCB003 (see FIGS. 8 to 12 for structure information). More preferably, the compound is TSB065 or TCB001.

The present invention is not to be limited in scope by the specific embodiments described herein, which are intended for the purpose of exemplification only. Functionally equivalent products, compositions and methods are clearly within the scope of the invention as described herein.

The compounds of the present invention are synthesised using the Heck Cross Coupling method.

Generally, the pathways for synthesis are as shown in FIGS. 1 and 2—the synthesis of the target compounds through the Heck Cross coupling approach. The reagents and reaction conditions for the chemical transformations shown in these figures are denoted by a letter for each reaction. The key for this labelling is:

For FIG. 1: (a). Pd₂(dba)₃/[(t-Bu)₃PH]BF₄, Tetrahydrofuran (“THF”), reflux, 17 h (82-97%) (b) LiOH, H₂O/EtOH, reflux, 3 hrs (c) Pd/C—H₂, CH₂Cl₂/EtOH, 17 h (d) LiOH, H₂O/EtOH, reflux, 3 hrs

For FIG. 2: (a) NaOMe, MeOH, 22, rt. 17 h, 84% (b) Pd/C (10% w/w), H₂, 1:1 CH₂Cl₂/MeOH, 17 h, rt, 93% (c) LION, MeOH/H₂O, reflux, 97% (d) Pd₂(dba)₃.CHCl₃, [(t-Bu)₃PH]BF₄, Cy₂NMe, THF, reflux, 17 h, 84% from 26, 72 h, 29% from 27 (e) LiOH, 9:1 EtOH/H₂O, reflux, 17 h, 66% (f) Pd/C, H₂, 1:1 CH₂Cl₂/EtOH, 17 h, 96% (g) LION, EtOH/H₂O, reflux, 17 h, 82%.

General Conditions

Terephthaldehyde, tributylphosphonium tetrafluoroborate, 1,3,5-tribromobenzene and 1,2,4-tribromobenzene were purchased from the Sigma-Aldrich Chemical Company. Dry THF was distilled from sodium benzophenone ketyl radical and stored over a sodium mirror. N-Methyldicyclohexylamine was distilled under reduced pressure and stored under argon. Dess-Martin Periodinane, Herrmann-Beller paladacycle [Herrmann et al. Chem. Int. Ed. Engl. 1995, 34, 1844-1849] (methyl 4-carboxybenzyl)triphenylphosphonium bromide, Pd₂(dba)₃.CHCl₃, and Pd(PPh₃)₄were prepared as described previously [Ukai et al. Organomet. Chem. 1974, 65, 253-266; Coulson, D. R. Inorg. Synth. 1972, 13, 121-123]. 1,3-di(hydroxymethyl)benzene was prepared by the LiAlH₄reduction of dimethyl 1,3-benzenedicarboxylate in a similar procedure to the 1,2-isomer [Sharpless, K. B.; Oi, R. Org. Synth. 1996, 73, 1-13] Ethyl 4-vinylbenzoate was prepared by the Fischer esterification of 4-vinyl benzoic acid [Broos et al. J. Chem. Ed. 1978, 55, (12), 813. 33; Tullen et al. J. Chem. Ed. 1971, 48, (7)].

NMR spectra were acquired on either a Bruker AV500 (¹H at 500.13 MHz,¹³C at 125.8 MHz) or a Bruker AV600 (¹H at 600.13 MHz,¹³C at 150.9 MHz) and all signals δ are reported in parts per million (ppm).¹H and¹³C assignments were made with the aid of DEPT, COSY, HSQC and HMBC sequences where appropriate.¹H spectra were referenced to residual (partially) undeuterated solvents, CDCl₃(CHCl₃at 7.26 ppm) and d₆-DMSO (d₅-DMSO at 2.50 ppm (pentet)).¹³C spectra were referenced to the deuterated solvents, CDCl₃at 77.16 ppm and d₆-DMSO at 39.52 ppm.

Infrared spectra samples were prepare using the KBr disc method and samples acquired on a Perkin Elmer. Spectrum One spectrometer at 2 cm⁻¹resolution. Electronic spectra were collected using a HP8452 spectrophotometer in 1 cm quartz cells at ˜1×10⁻⁵or ˜1×10⁻⁶MolL⁻¹in the solvents indicated. Fluorescence spectra were recorded at 1×10⁻⁷MolL⁻¹.

Mass spectra were acquired on a VG Autospec employing the electron impact (EI) ionization mode.

Standard Conditions for Heck Cross-Coupling Procedure

To a flame-dried schienk flask was added the halobenzene (1 equiv), Pd₂(dba)₃.CHCl₃(2-15 mol %) and [(t-Bu)₃PH]BF₄(10-60 mol %) which were subsequently dried under vacuum for 15 minutes before being dissolved in dry THF. N-Methyldicyclohexylamine (4 equiv) and ethyl 4-vinylbenzoate 7 (3.3 equiv) were added via syringe and the reaction monitored by TLC (neat CH₂Cl₂). Upon completion of the reaction the residual THF was removed in vacuo, the crude material redissolved in CH₂Cl₂and filtered to remove any insoluble material before being absorbed onto fine silica and eluting with 0:100 to 2:98 MeOH/CH₂Cl₂.

Standard Method for Reduction of Alkenes

The alkene was loaded into a glass autoclave tube and dissolved/suspended in 1:1 CH₂Cl₂/MeOH or 1:1 CH₂Cl₂/EtOH depending upon the ester present. Argon was bubbled through the mixture for 10 minutes before 10% Pd/C (˜10 wt % of alkene) was added, and the flask pressurized with H₂(50 atm). The reaction was allowed to proceed for 17 h before being depressurized, purged with argon, filtered through a pad of celite and concentrated under reduced pressure. Further purification is described for each compound when necessary.

Standard Method for Saponification Reactions

The ester (1 equiv) and LiOH (2 equiv per ester) were dissolved in 9:1 H₂O/MeOH or H₂O/EtOH depending upon the ester and refluxed overnight. After cooling to room temperature the solvent was removed under reduced pressure, the remaining solution diluted with H₂O, cooled in an ice-bath and the pH adjusted to 3 by the addition of HCl (1M). The precipitate was collected filtered and product dried under vacuum.

Prepared as per, the standard procedure using 1,3,5-tribromobenzene 8 (1010 mg, 3.21 mmol), Pd₂(dba)₃.CHCl₃(882 mg, 0.85 mmol), t-Bu₃PHBF₄(560 mg, 1.93 mmol), Cy₂NMe (3.0 mL), ethyl 4-vinylbenzoate 7 (1870 mg, 10.61 mmol) and THF (40 mL). The product was eluted with 2:98 MeOH/CH₂Cl₂and recrystallized from CH₂Cl₂/EtOH to give 10 as an off white powder, 1.86 g (97%).

¹H NMR (600 MHz, CDCl₃): δ1.42 (t, J=7.1 Hz, 9H, CH₃), 4.40 (q, J=7.1 Hz, 6H, CH₂), 7.24 (AB quartet, 6H, vinyl), 7.60 (d, J=8.3 Hz, 6H, ArH), 7.61 (s, 3H, ArH), 8.06 (d, J=8.3 Hz, 6H, ArH).

¹³C NMR (150 MHz, CDCl₃): δ 14.51 (CH₃), 61.13 (CH₂), 125.0 (CH), 126.5 (CH), 128.7 (CH), 129.7 (C), 130.2 (CH), 130.5 (CH), 137.9 (C), 141.5 (C), 166.5 (C═O).

IR (KBr): ν (cm⁻¹) 2979, 2929, 1713, 1604, 1279, 1178, 1105, 762, 698

HR-EI⁺-MS: C₃₉H₃₆O₆requires 600.2512 amu. found 600.2513.

EI⁺-MS: MI=C₃₉H₃₆O₆; m/z: 600.3 (100%)=MI⁺, 555.2 (7%)=[MI-EtO]⁺.

UV-Vis (CH₂Cl₂): λ (nm) [log ε(M⁻¹cm⁻¹)] 258 [4.49], 330 [4.28]

Fluorescence (CH₂Cl₂): excitation (nm) [emission (nm)] 258 [397, 418, 518], 330 [397, 418]; (cyclohexane) 328 [393, 413]

Using the standard saponification procedure, 10 (252.1 mg, 0.42 mmol), LiOH.H₂O (112.0 mg, 2.7 mmol) and 1:9 H₂O/EtOH (20 mL) gave an gelatinous precipitate that was collected and recrystallised from THF/H₂O and dried to give the triacid 5 as a pale brown powder, 209 mg (95%).

¹H, NMR (500.1 MHz, d₆-DMSO): δ 7.49 (m, 6H, vinyl CH), 7.76 (d, J=8.5 Hz, 6H, ArH), 7.88 (s, 3H, core ArH), 7.98 (d, J=8.5 Hz, 6H, ArH).

¹³C NMR (125.8 MHz, d₆-DMSO): δ (ppm) 125.0, 126.5, 128.4, 129.7, 129.9, 130.50, 137.6, 141.3, 167.1.

IR (KBr): ν (cm⁻¹) 3067, 3026, 1684 (ν_C═O), 1604, 1566, 1420, 1384, 1312, 1286, 1179.

HR-EI⁺-MS: C₃₃H₂₄O₆requires 516.1573 amu. found 516.1564.

EI⁺-MS: MI=C₃₃H₂₄O₆; m/z: 516.1 (100%)=MI⁺, 472.1 (11.3%)=[MI-CO₂]⁺.

Conducted as per the standard reduction procedure with trimester 10 (251 mg, 0.42), Pd/C (20 mg) and 1:1 CH₂Cl₂/EtOH (15 mL). The crude product was recrystallized from CH₂Cl₂/EtOH to give the triester 11 237 mg (93%) of a white solid.

¹H-NMR (600 MHz, CDCl₃): δ 1.38 (t, J=7.1 Hz, 9H, CH₃), 2.87 (m, 12H, bridge CH₂), 4.36 (q, J=7.1 Hz, 6H, CH₂), 6.76 (s, 3H), 7.20 (d, J=8.1 Hz, 6H, ArH), 7.97 (d, J=8.1 Hz, 6H, ArH).

¹³C NMR (150 MHz, CDCl₃): δ 14.4, 37.5, 38.0, 60.8, 126.5, 128.3, 128.6, 129.7, 141.4, 147.2, 166.7.

HR-EI-MS: C₃₉H₄₂O₆requires 606.2981 amu. found 606.2994.

Using the standard procedure triester 11 (252.0 mg, 0.42 mmol), LiOH.H₂O (107.2 mg, 2.6 mmol) and 1:9 H₂O/EtOH (20 mL) gave triacid 3 202 mg (93%) as a white powder.

¹H-NMR (500 MHz, d₆-DMSO): δ 2.82 (cm, 12H, CH₂), 6.83 (s, 3H, ArH), 7.28 (d, J=8.2 Hz, 6H, ArH), 7.85 (d, J=8.2 Hz, 6H, ArH).

¹³C NMR (125 MHz, CDCl₃): δ 36.7, 37.1, 126.2, 128.4, 126.2, 129.3, 140.9, 146.9, 167.3.

IR (KBr): ν (cm⁻¹) 3067, 2929, 1686, 1610, 1422, 1315, 1288, 1179.

HR-EI⁺-MS: C₃₃H₃₀O₆requires 522.2042 amu. found 522.5897.

EI⁺-MS: MI⁺=C₃₃H₃₀O₆; m/z: 504.2 (90%)=[MI-H₂O]⁺, 387.1 (100%)=[MI-CH₂(C₆H₄CO₂H)]⁺.

Using the standard Heck cross-coupling procedure, 1,2,4-tribromobenzene 13 (1.018 g, 3.2 mmol), ethyl 4-vinylbenzoate 9 (1.843 g, 10.5 mmol), Pd₂(dba)₃.CHCl₃(87.6 mg, 0.08 mmol), [(t-Bu)₃PH]BF₄(122.6 mg, 0.42 mmol), Cy₂NMe (3 mL) in THF (40 mL) were heated for 17 h. The crude mixture was subjected to flash chromatography eluting with neat CH₂Cl₂. The crude product was recrystallised from CH₂Cl₂/EtOH to give 1.681 g (88%) of a pale yellow solid.

¹H NMR (600 MHz, CDCl₃): δ 1.409, 1.413, 1.416 (3×t, J=7.1 Hz, 9H, CH₃), 4.392, 4.394, 4.400 (3×q, J=7.1 Hz, 6H, CH₂) 7.06-7.11 (m, 2H, vinyl CH), 7.17-7.28 (m, 2H, vinyl CH), 7.51-7.67 (cm, 9H), 7.73 (m, 1H, core ArH), 8.04-8.09 (cm, 6H, ArH).

¹³C NMR (150 MHz, CDCl₃): δ 14.5 (CH₃), 61.1, 61.13, 61.1 (CH₂), 125.8 (CH), 126.5 (CH), 126.5 (CH), 126.6 (CH), 126.4 (CH), 127.3 (CH), 128.3 (CH), 128.4 (CH), 128.7 (CH), 129.6 (C), 129.8 (C), 129.8 (C), 130.2 (CH), 130.2 (CH), 130.3 (CH), 130.5 (C), 130.7 (C), 131.3 (C), 135.6 (C), 136.4 (C), 136.9 (C), 141.62 (C═O), 141.66 (C═O), 141.7 (C═O).

IR (KBr): ν (cm⁻¹) 2981, 1713 (ν_C═O), 1604, 1278, 1178, 1107

HR-EI⁺-MS: C₃₉H₃₆O₆requires 600.2512 amu. found 600.2504.

EI⁺-MS: MI=C₃₉H₃₆O₆; m/z: 600.2 (100%)=MI⁺, 555.2 (13.3%)=[MI-EtO]⁺, 437.1 (70.1%)=[MI-2×OEt-EtO₂CH]⁺

UV-Vis (Solv): λ (nm) [E, (M⁻¹cm⁻¹)] 258 [4.53], 338 [4.83], 362 [4.76, shoulder].

Fluorescence (CH₂Cl₂): excitation (nm) [emission (nm)] 258 [436 (shoulder), 450], 338 [436 (shoulder), 450]; (cyclohexane) 334 [416, 440]

Triester 14 (250 mg, 0.42 mmol) and LiOH.H₂O (70.2 mg, 1.67 mmol) in 9:1 EtOH/H₂O were treated as described in the general saponifiaction procedure giving the triacid 6 186.6 mg (86%) as a yellow/brown solid.

IR (KBr): ν (cm⁻¹) 2929, 1684 (ν_C═O), 1603, 1419, 1315, 1287, 1178, 1125, 763.

Triester 14 (306 mg, 0.51 mmol) and Pd/C (10% w/w, ca 40 mg) in 1:1 EtOH/CH₂Cl₂(20 mL) was treated as described. The crude product was recrystallised from THF and hexane to give ester 15 305 mg (98%).

¹H-NMR (600 MHz, CDCl₃): δ 1.376, 1.381, 1.393 (3×t, 3×CH₃, 9H) 2.79-2.95 (m, 12H), 4.33-4.40 (m, 6H), 6.86 (s, 1H), 6.95 (d, J=7.8 Hz, 1H), 7.04 (d, J=7.8 Hz, 1H), 7.17 (AB d, J=8.0 Hz, 2H), 7.19 (AB d, J=8.0 Hz, 2H) 7.21 (AB d, J=8.1 Hz, 2H), 7.95 (cm, J=7.7 Hz, 6H)

¹³C NMR (150 MHz, CDCl₃): δ 14.48, 14.50, 34.0, 34.4, 37.2, 37.7, 38.1, 61.0, 61.0, 126.6, 128.43, 128.54, 128.56, 128.7, 129.5, 129.7, 129.8, 129.9, 136.7, 139.0, 139.3, 147.2, 147.2, 147.3, 166.7, 166.8, 166.8.

IR (KBr): ν (cm⁻¹) 2981, 2942, 1713, 1610, 1283, 1177, 1123, 1108.

HR-EI⁺-MS: C₃₉H₄₂O₆requires 606.2981 amu. found EI⁺-MS: MI=C₃₉H₄₂O₆; m/z: (%)=MI⁺, (%)=[MI-]⁺

Triester 15 (200 mg, 0.33 mmol) and LiOH.H₂O (92.2 mg, 2.15 mmol) in 9:1 EtOH/H₂O (25 mL) was treated as described in the general saponification procedure, giving triacid 16 161 mg (94%).

¹H NMR (500.1 MHz, d₆-DMSO): δ 2.77-2.91 (m, 12H, methylene), 6.960 (AB, J=8.5 Hz, 1H), 6.967 (s, 1H), 7.07 (AB, J=8.5 Hz, 1H), 7.271 (AB, J=8.4 Hz, 2H), 7.296 (AB, J=8.4 Hz, 2H), 7.299 (AB, J=8.4 Hz, 2H), 7.82-7.87 (m, 6H), 12.83 (br s, CO₂H).

¹³C NMR (125.8 MHz, d₆-DMSO): δ 33.1, 33.5, 36.3, 36.7, 37.0, 126.2, 128.5, 128.6, 128.6, 128.6, 129:0, 129.3, 129.4, 136.4, 138.6, 138.7, 146.9, 146.9, 167.3, 167.3.

IR (KBr): ν (cm⁻¹), 1688, 1610, 1422, 1315, 1289, 1178.

HR-EI⁺-MS: C₃₃H₃₀O₆requires 522.2042 amu. found 522.2045.

EI⁺-MS: MI=C₃₃H₃₀O₆; m/z: 522.2 (9%)=MI⁺, 504.2 (86%)=[MI-H₂O]⁺, 387.1 (100%)=[MI-CH₂(C₆H₄CO₂H)]⁺.

1,2,4,5-tetrabromobenzene 17 (255.8 mg, 0.66 mmol), ethyl-4-vinylbenzoate 9 (498.6 mg, 2.83 mmol), Pd₂(dba)₃.CHCl₃(69.2 mg, 0.07 mmol), [(t-Bu)₃PH]BF₄(75.4 mg, 0.26 mmol), Cy2NMe (0.8 mL) in THF (8 mL) were treated under the aforementioned cross-coupling procedure. The product was purified by flash chromatography with CH₂Cl₂/MeOH (100:0-99:1) an the eluent. The crude material was recrystallized from CH₂Cl₂/EtOH to give triester 18 541.2 mg (82%) as a bright yellow solid.

¹H NMR (600 MHz, CDCl₃): δ 1.41 (t, J=7.1 Hz, 12H, CH₃), 4.40 (q, J=7.1 Hz, 8H, CH₂), 7.13 (d, J=16 Hz, 4H, vinyl CH), 7.56 (d, J=16.1 Hz, 4H, vinyl CH), 7.60 (d, J=8.3 Hz, 8H, ArH), 7.83 (s, 2H, core ArH), 8.06 (d, J=8.3 Hz, 4H, ArH).

¹³C NMR (150 MHz, CDCl₃): δ 14.3 (CH₃), 61.0 (CH₂), 125.2 (ArCH, core), 126.5 (ArCH), 128.1 (CH, vinyl), 129.7 (C_q), 130.1 (ArCH), 131.0 (CH, vinyl), 135.6 (C), 141.4 (C), 166.3 (C═O).

IR (KBr): ν (cm⁻¹) 2981, 1710, 1637, 1617, 1604, 1282, 1180, 1107.

HR-EI⁺-MS: C₅₀H₄₆O₈required 774.3193 amu. found 774.3506 amu.

EI⁺-MS: MI=C₅₀H₄₆O₈; m/z: 774.4 (100%)=MI⁺, 729.1 (16%)=[MI-OEt]⁺, 611.1 (45%)=[MI-CH₂(C₆H₄CO₂Et)]⁺.

UV-Vis (Solv): λ (nm) [log ε(M⁻¹cm⁻¹)] 258 [4.62], 312 [4.73], 346 [4.82].

Fluorescence (CH₂Cl₂): excitation (nm) [emission (nm)] 258 [455, 518], 312 [362, 381, 452], 346 [381, 454]; (cyclohexane) 336 [442].

Triester 18 (151.7 mg, 0.20 mmol), LiOH.H₂O (86.6 mg, 2.06 mmol) in 9:1 EtOH/H₂O (30 mL) were treated as described in the general saponification procedure. The crude product was recrystallized from THF and MeOH to give triacid 7 111.3 mg (85%) as a tan powder.

¹H NMR (500 MHz, CDCl₃): δ (d, J=Hz, 6H, ArH), (s, 3H, ArH), (d, J=Hz, 6H, ArH)

¹³C NMR (125.8 MHz, CDCl₃): δ (ppm), 124.2, 127.1, 127.6 (CH), 129.5, 129.9, 130.7, 135.3, 141.5, 167.2.

IR (KBr): ν (cm⁻¹)

HR-EI⁺-MS: C₄₂H₃₀O₈requires 662.1941 amu,

Triester 18 (200.4 mg, 0.24 mmol) and Pd/C (10% w/w, c.a. 30 mg) in 1:1 EtOH/CH₂Cl₂(40 mL) was treated as described. The crude product was recrystallized from CH₂Cl₂and EtOH to give compound 19147.1 mg (79%) as a white solid.

¹H NMR (600 MHz, CDCl₃): δ 1.38 (t, J=7.2 Hz, 12H), 2.82 (m, 16H), 4.36 (q, J=7.2 Hz, 8H), 6.81 (s, 2H), 7.18 (d, J=8.0 Hz, 8H, ArH), (s, 3H, ArH), (d, J=Hz, 6H, ArH).

¹³C NMR (150 MHz, CDCl₃): δ 14.8, 34.0, 37.8, 61.0, 128.5, 128.6, 129.8, 130.6, 136.9, 147.2, 166.7.

IR (KBr): ν (cm⁻¹) 2980, 2935, 1716, 1611, 1285, 1176, 1107, 1022.

HR-EI⁺-MS: C₅₀H₅₄O₈requires 782.3819 amu,

Triester 19 (101.4 mg, 0.13 mmol), LiOH.H₂O (51 mg, 1.22 mmol) in 9:1 EtOH/H₂O (25 mL) were treated as described in the general saponification procedure, to give tetraacid 20 87.3 mg (97%) as a white solid.

¹H NMR (500.1 MHz, d₆-DMSO): δ 2.77 (s, 16H, CH₂CH₂), 6.86 (s, 2H, H2), 7.26 (AB, J=8.3 Hz, 8H, H2′) 7.84 (AB, J=8.3 Hz, 8H, H3′).

¹³C NMR (125.8 MHz, d₆-DMSO): δ 33.1, 36.8, 128.5, 128.6, 129.4, 136.3, 147.0, 167.3.

IR (KBr): ν (cm⁻¹) 2945, 2863, 1688, 1610, 1422, 1315, 1288, 1178.

HR-EI⁺-MS: C₄₂H₃₈O₈requires 670.2567 amu. found 670.2562.

EI⁺-MS: MI=C₄₂H₃₈O₈; m/z: 517.0 (100%)=[MI-H₂O—(CH₂(C₆H₄CO₂H)]⁺, 499.0 (12%)=[MI-2(H₂O)—(CH₂(C₆H₄CO₂H)]⁺, 381.0 (19.5%)=[MI-H₂O-2(CH₂(C₆H₄CO₂H)]⁺, 135.0=[(CH₂(C₆H₄CO₂H)]⁺.

Method A:

1,4-Dibromobenzene 26 (101.5 mg, 0.43 mmol), ethyl 4-vinyl benzoate 7 (166 mg, 0.94 mmol), Pd₂(dba)₃.CHCl₃(45.1 mg, 0.04 mmol), [(t-Bu)₃PH]BF₄(52.2 mg, 0.18 mmol), Cy₂NMe (300 □l) in THF (5 mL) were heated at reflux overnight. The THF was removed under reduced pressure, the crude product purified using flash chromatography with CH₂Cl₂as the eluent. Additional recrystallisation from CH₂Cl₂and EtOH, gave 28 152.2 mg (84%).

Method B:

1,4-dichlorobenzene 27 (59.9 mg, 0.41 mmol), ethyl 4-vinyl benzoate 7 (160 mg, 0.91 mmol), Pd₂(dba)₃.CHCl₃(42.1 mg, 0.04 mmol), [(t-Bu)₃PH]BF₄(47:7 mg, 0.16 mmol), Cy₂NMe (300 DI) in THF (5 mL) were heated at reflux for 3 days. The reaction mixture was concentrated under reduced pressure, the crude product was purified by chromatography with CH₂Cl₂as the eluent. Additional recrystallisation from CH₂Cl₂and EtOH, gave 28 49.8 mg (29%).

¹H NMR (600 MHz, CDCl₃): δ 1.41 (t, J=7.1 Hz, 6H), 4.39 (q, J=7.1 Hz, 4H), 7.14 (AB, J=16.3 Hz, 2H, vinyl CH), 7.24 (AB, J=16.3 Hz, 2H, vinyl CH), 7.55 (s, 4H), 7.57 (d, J=8.1 Hz, 4H), 8.04 (d, J=8.1 Hz, 4H).

¹³C NMR (150 MHz, CDCl₃): δ 14.5 (CH₃), 61.1 (CH₂), 126.5 (CH), 127.4 (CH), 128.0 (CH), 129.5 (C), 130.2 (CH), 130.7 (CH), 136.9 (C), 141.8 (C), 166.5 (C═O).

IR (KBr): ν (cm⁻¹) 2984, 2925, 1716 (C═O), 1708 (C═O), 1279, 1179, 1107.

HR-EI⁺-MS: C₂₈H₂₆O₄requires 426.1831 amu. found 426.1824 UV-Vis (Solv): λ (nm) [log ε(M⁻¹cm⁻¹)] 254 [3.85], 372 [4.74].

Fluorescence (CH₂Cl₂): excitation (nm) [emission (nm)] 254 [414, 437], 372 [414, 437]; (cyclohexane) 328 [393, 413].

Using the standard procedure 28 (150.7 mg, 0.35 mmol), LiOH.H₂O (62.3 mg, 1.48 mmol) and 1:9 H₂O/EtOH (20 mL) gave an impure dark brown/black precipitate. The crude product was recrystallized from H₂O and EtOH to give acid 3 as a dark brown powder 86.2 mg (66%).

HR-EI⁺-MS: C₂₄H₁₈O₄requires 370.1205 amu. found 370.1205.

(Methyl 4-carboxybenzyl)triphenylphosphonium bromide (22) (4.43 g, 9.02 mmol) was dissolved in MeOH (100 mL) and treated with NaOMe (45 mL 0.222M in MeOH). The ensuing yellow solution was treated with terephthalaldehyde (512 mg, 3.82 mmol) in one portion and the resultant mixture was heated at reflux for 17 h. The resulting yellow precipitate formed was collected and washed with MeOH to give 23 1.27 g (84%). 40:60 mixture of the E/E and E/Z products.

¹H NMR (500.1 MHz, CDCl₃): δ 3.834 (s, CH₃, EE), 3.837 (s; CH₃, EZ), 3.85 (s, CH₃, EZ), 6.691 (AB, J=12.3 Hz, vinyl CH, EE), 6.726 (AB, J=12.3 Hz, vinyl CH, EZ), 6.735 (AB, J=12.3 Hz, vinyl CH, EE), 6.774 (AB, J=12.3 Hz, vinyl CH, EZ), 7.10 (s, core ArH, EE), 7.23 (AB, J=8.3 Hz, ArH, EZ), 7.326 (AB, J=16.4 Hz, vinyl CH, EZ), 7.345 (AB, J=8.4 Hz, ArH, EE), 7.389 (AB, J=16.4 Hz, vinyl CH, EZ), 7.393 (AB, J=8.4 Hz, ArH, EZ), 7.54 (AB, J=8.3 Hz, ArH, EZ), 7.72 (AB, J=8.5 Hz, ArH, EZ), 7.84 (AB, J=8.4 Hz, ArH, EE), 7.87 (AB, J=8.4 Hz, ArH, EZ), 7.85 (AB, J=8.5 Hz, ArH, EZ).

¹³C NMR (125.8 MHz, CDCl₃): δ 52.1 (CH₃), 52.1 (CH₃), 126.6 (CH), 126.9 (CH), 127.6 (CH), 128.2 (C), 128.2 (C), 128.3 (C), 128.7 (CH), 128.8 (CH), 129.1 (CH), 129.2 (CH), 129.3 (CH), 129.4 (CH), 129.6 (CH), 130.7 (CH), 131.6 (CH), 135.6 (C), 136.0 (C), 136.1 (C), 141.8 (C), 141.8 (C), 142.0 (C).

IR (KBr): ν (cm⁻¹) 3011, 2959, 1716, 1606, 1436, 1276, 1182, 1109.

HR-EI-MS: C₂₆H₂₂O₄requires 398.1518 amu. found 398.1515 amu.

Diester 23 (1.27 g, 3.19 mmol) and Pd/C (10% w/w c.a 100 mg) in 1:1 MeOH/CH₂Cl₂(30 mL) was treated as described in the general procedure section. The crude product was recrystallized from CH₂Cl₂and MeOH to give 1.20 g (93%) as a white solid.

¹H NMR (600 MHz, CDCl₃): δ 2.93 (AA′BB′, 8H, CH₂CH₂), 3.91 (s, 6H, OCH₃), 7.05 (s, 4H, H2/H3), 7.21 (d, J=7.6 Hz, 4H, H2′), 7.95 (d, J=7.6 Hz, 4H, H3′).

¹³C NMR (150 MHz, CDCl₃): δ (ppm) 37.2, 38.0, 52.1, 128.1, 128.6, 128.7, 129.8, 139.0, 147.3, 167.3.

IR (KBr): ν (cm⁻¹) 2944, 2923, 1726, 1609, 1431, 1280, 1176, 1105.

HR-EI⁺-MS: C₂₆H₂₆O₄requires 402.831 amu. found 402.1834 amu.

EI⁺-MS: MI=C₂₆H₂₆O₄; m/z: 402.2 (8%)=MI⁺, 370.1 (33.2%)=[MI-MeOH]⁺, 253.1 (100%)=[MI-CH₂C₆H₄CO₂Me]⁺, 149.1=[CH₂C₆H₄CO₂Me]⁺.

Method 1:

Diester 24 (308.6 mg, 0.77 mmol), LiOH.H₂O (125 mg, 3.0 mmol) in MeOH/H₂O (9:1, 20 mL) were treated as described in the general saponification procedure, to provide diacid 25 280 mg (97%).

Method 2:

Diacid 3 (107.2 mg, 0.25 mmol) and Pd/C (10% w/w c.a. 10 mg) in 1:1 EtOH/CH₂Cl₂(10 mL) was treated as described. The crude mixture containing 29 (103 mg, 96%) was suspended in 9:1 EtOH/H₂O (10 mL) and LiOH.H₂O (26.3 mg, 0.63 mmol) added and treated under the general saponification procedure described earlier to afford diacid 3 (73.2 mg, 82%)

¹H NMR (500.1 MHz, d₆-DMSO): δ 2.88 (AA′BB′, 8H, CH₂CH₂), 7.11 (s, 4H, H2/H3), 7.32 (d, J=8.2 Hz, 6H, ArH), 7.85 (d, J=8.2 Hz, 6H, ArH).

¹³C NMR (125.8 MHz, d₆-DMSO): δ 36.2, 37.0, 128.3, 128.6, 129.3, 138.7, 146.8, 167.3.

IR (KBr): ν (cm⁻¹) 2944, 2923, 1685 (C═O), 1610, 1425, 1318, 1292, 1180, 537.

HR-EI⁺-MS: C₂₄H₂₂O4 requires 374.1518 amu.

Isophthaldehyde 30 (460 mg, 3.43 mmol), xx (4.4 g, 8.95 mmol), NaOMe (20 mL, 1.0 M) in MeOH (100 mL) were treated under analogous conditions to those described for the preparation of alkene 23. The white precipitate was filtered, washed with MeOH, and dried to give 4 (0.85 g, 62%). The product was a 44:56 mixture of the E/E and E/Z products.

¹H NMR (600.1 MHz, CDCl₃): δ 3.82 (s, CH₃, ct), 3.83 (s, CH₃, E/E), 3.85 (s, E/Z), 6.64 (AB, J=12.4 Hz, trans vinyl CH, E/E), 6.67 (AB, J=12.4 Hz, trans vinyl CH, E/E), 6.76 (AB, J=12.3 Hz, trans vinyl CH, E/Z), 6.81 (AB, J=12.3 Hz, trans vinyl CH, E/Z), 7.05 (dd, J₁=7.6 Hz, J₂=1.4 Hz, H4, ee), 7.08 (s, H2, ee), 7.10 (d, J=7.7 Hz, H4/H6, E/Z) 7.13 (AB, J=16.5 Hz, cis vinyl CH, E/Z), 7.17 (t, J=7.6 Hz, H5, E/E), 7.25 (AB, J=8.2 Hz, H2′, E/Z), 7.27 (t, J=7.7 Hz, H5, E/Z), 7.33 (AB, J=16.5 Hz, cis vinyl CH, E/Z), 7.36 (AB, J=8.2 Hz, ArH, E/Z), 7.46 (s, H2, E/Z), 7.50 (d, J=7.7 Hz, H4/H6, E/Z), 7.66 (AB, J=8.3 Hz, ArH, E/Z), 7.76 (AB, J=8.4 Hz, H3′, E/E), 7.84 (AB, J=8.3 Hz, ArH, E/Z), 7.92 (AB, J=8.4 Hz, ArH, E/Z).

¹³C NMR (150.9 MHz, CDCl₃): δ 52.1 (CH₃), 52.1 (CH₃), 126.2 (CH), 126.6 (CH), 127.0 (CH), 127.6 (CH), 127.9 (CH), 128.1 (C), 128.2 (C), 128.3 (C), 128.3 (CH), 128.3 (CH), 128.7 (CH), 128.9 (CH), 128.9 (CH), 129.2 (CH), 129.3 (CH), 129.3 (CH), 129.5 (CH), 129.6 (CH), 130.9 (CH), 131.7 (CH), 131.8 (CH), 136.6 (C), 136.8 (C), 136.8 (C), 141.6 (C), 141.9 (C), 165.9 (C), 165.9 (C), 166.0 (C).

IR (KBr): ν (cm⁻¹) 1720, 1606, 1435, 1280, 1179, 1109.

HR-EI⁺-MS: C₂₆H₂₂O₄requires 398.1518 amu. found 398.1518

The E/Z isomeric mixture 4 (499.3 mg, 1.25 mmol) and Pd/C (10% w/w, c.a. ˜50 mg) in MeOH/CH₂Cl₂(40 mL, 1:1) was treated as described previously. The crude product was recrystallized from CH₂Cl₂/MeOH to give ester 31 (489.7 mg, 98%) as a white solid.

¹H-NMR (600 MHz, CDCl₃): δ 2.8-2.96 (AA′BB′, 8H, CH₂CH₂), 3.90 (s, 6H, Me), 6.91 (s, 1H, H2), 6.99 (dd, 2H, J=7.6 and 1.4 Hz, 2H, H4/H6), 7.16-7.25 (cm, 5H, ArH), 7.96 (d, J=8.2 Hz, 4H, H3′).

¹³C NMR (150 MHz, CDCl₃): δ 37.5, 38.0, 52.1, 126.3, 128.0, 128.5, 128.7, 128.8, 129.8, 141.3, 147.3, 167.2.

IR (KBr): ν (cm⁻¹) 1715, 1607 (m), 1438, 1279, 1109.

HR-EI⁺-MS: C₂₆H₂₆O₄requires 402.1831 amu. found 402.1840.

Ester 25 (202.6 mg, 0.50 mmol), LiOH.H₂O (92.2 mg, 2.20 mmol) and 9:1 MeOH/H₂O (30 mL) were treated as described in the general saponfication procedure, giving 175.8 mg (94%) as a white solid.

¹H-NMR (500.1 MHz, d₆-DMSO): δ 2.87 (AA′BB′, 8H, CH₂CH₂), 6.99-7.05 (m, 3H, ArH) 7.15 (t, J=7.4 Hz, 1H, H5), 7.31 (AB, J=8.4 Hz, 4H, H2′), 7.84 (AB, J=8.4 Hz, 4H, H3′), 12.8 (br s, 2H, CO₂H).

¹³C NMR (125.8 MHz, d₆-DMSO): δ 36.5, 37.0, 126.0, 128.1, 128.4, 128.6, 128.6, 129.3, 141.0, 146.9, 167.3.

HR-EI⁺-MS: C₂₄H₂₂O₄requires 374.1518 amu. found 374.1523.

EI⁺-MS: MI=C₂₄H₂₂O₄; m/z: (%)=MI⁺, 239.0 (100%)=[MI-(CH₂(C₆H₄CO₂H)]⁺, 193.0 (34%)=[MI-(CH₂(C₆H₄CO₂H)—CO₂H—H⁺]⁺.

(A) Haemolytic Activity

In a preliminary study using disk diffusion methodology, a compound of Formula C (TSB007 also known as “135B”) (10 mg/ml) had antibacterial activity mostly against Gram-positive bacteria.

As an indicator of toxicity to human cells, haemolysis experiments on sheep erythrocytes were undertaken with TSB007.

10 mg/ml stock solution of TSB007 was prepared in 100% DMSO. Master stock solutions of TSB007 (10 mg/ml) were made by dissolving 20 mg dehydrated compound in 2 ml 100% DMSO. They were stored in foil-covered glass bottles at −20° C. Master stocks stored in this way retained full antimicrobial activity for a minimum period of 6 weeks (results not shown).

Serial, 10-fold dilutions were performed in PBS to make solutions of 1000, 100, 10 and 1 mg/L of TSB007. In microcentrifuge tubes, 500 μl of each dilution was combined with 480 μl PBS and 20 μl washed sheep erythrocytes (100%) so that the final concentration of erythrocytes was 2% and the final concentrations of compound were 500, 50, 5 and 0.5 mg/L. Dilutions of DMSO without. TSB007 were prepared and tested as above to check for haemolysis due to DMSO. A 100% haemolysis control was prepared with 980 μl water and 20 μl erythrocyte suspension. A negative control was prepared with 980 μl PBS and 20 μl erythrocyte suspension. Dilutions and controls were prepared and tested in duplicate.

Tubes were incubated at 37° C. for 2 h on a rocker then centrifuged at 12 000 g for 5 mins. The OD₅₄₀of the supernatant was determined using 100 μl volumes transferred to a microtitre tray. % haemolysis was determined by blanking the OD against that of the negative control and presenting the resulting OD as a proportion of the OD of the positive control (blanked with water).

The haemolytic activity of TSB007 was very low (Table 1). No haemolysis was observed for the equivalent tests of DMSO.

L929 cells grown to approximately 80% confluency in HGM-M were washed with Hanks, trypsonised to detach the cells from the flask, then diluted to 10⁵cells/ml in HGM-M and 200 μl used to inoculate the wells of a 96-well microtitre tray. After incubation for 24 h at 37° C., adherent cells were washed with Hanks and then 100 μl HGM-M was added to the wells. Fresh TSB007 master stock solution (10 mg/ml) was serially 10-fold diluted in HGM-M to make solutions of 1000, 100, 10 and 1 μg/ml of TSB007. To the wells of the microtitre tray, 100 μl of these TSB007 solutions were also added, giving final concentrations of 500, 50, 5 and 0.5 μg/ml of TSB007. The same dilutions of DMSO were prepared and tested to check that cytotoxic activity was not due to the DMSO. A negative control was prepared containing only HGM-M, and a positive control was prepared by adding 100 μl of carboplatin (10 mg/ml; Mayne Pharma Pty Ltd, Australia) to wells containing 100 μl HGM-M. Controls and dilutions of TSB007 and DMSO were prepared in duplicate.

After 24 h shaking at 37° C., cytotoxicity was quantified using the neutral red assay. The cells were washed with Hanks then 200 μl HGM-M and 20 μl Neutral Red (3.3 g/L; Sigma-Aldrich) were added to each well. After 2 h shaking at 37° C., cells were washed twice with PBS then 200 μl of 1% acetic acid in 50% EtOH was added to each well to solubilise the stain. After 15 min shaking at 37° C. the OD_{690 nm}of the wells was measured and subtracted from the OD_{540 nm}. Results were then blanked against wells to which no cells had been added, and converted to a ratio of the OD of the negative control. Ratios ≦0.5 indicated a cytostatic effect. Testing was performed on two separate occasions.

At concentrations of 0.5-50 μg/ml, TSB007 was not significantly cytostatic to L929 cells (Table 2). The compound was cytostatic at 500 μg/ml. The concentrations of DMSO present in the different TSB007 dilutions were not significantly cytostatic (average ratios ≧0.515). The controls performed as expected.

To perform the Ames Test on TSB007, a method based on that published by Zeiger and Mortelmans in Current Protocols in Toxicology (1999; Section 3.1.1-3.1.29) was used, in the absence of a metabolic activation system.

Briefly, single colonies from overnight BA cultures of three commonly used Salmonella tester strains, S. typhimurium TA98, TA100 and TA1535, were used to inoculate 10 ml of nutrient broth. After incubation for 15-18 h at 37° C. with shaking, the concentration of the culture was appropriate for use in the test (˜1-2×10⁹cfu/ml).

Glucose minimal agar plates with a volume of 20 ml were dried thoroughly. Molten top agars (2 ml) were prepared, supplemented with biotin and trace histidine, and maintained at 43-48° C. To these, 50 μl of the bacterial broth culture (˜1×10⁹cells) and 100 μl of test solution (see, below) were added. The molten top agar was then poured directly over the surface of the glucose minimal agar and gently swirled to ensure even distribution of the agar. Once solidified, plates were incubated at 37° C. for 48 h then colony counts were performed. Plates were prepared in duplicate.

Test solutions were prepared as follows and included three concentrations of TSB007, a negative solvent control and a positive control (selected from the recommended positive control chemicals and test concentrations) for each strain. Master stock solution (10 mg/ml) was incorporated directly into molten top agar to test the compound at 1000 μg/plate. Dilutions of the stock were prepared in sterile distilled water to also test 100 μg/plate and 300 μg/plate. DMSO (100%) was incorporated into molten top agar as the negative solvent control. This was equivalent to the highest amount of DMSO incorporated into the molten top agar when testing TSB007. The positive controls used were 4-nitro-o-phenylenediamine at 2.5 μg/plate (for TA98), and sodium azide at 5 μg/plate (for TA100 and TA1535).

Colony counts that were two to three times greater than on the negative solvent control plate indicated a mutagenic effect and were regarded as ‘positive’. In these cases, the increase in colonies is usually dose related. A ‘positive’ result in this test is highly predictive of rodent carcinogenicity.

TSB007 gave a ‘negative’ result (Table 3). In general, the number of colonies on plates containing TSB007 was not greater than on the negative control plates. The positive control performed as expected. The decrease in colony counts at 1000 μg/plate TSB007 may be due to antimicrobial activity against the tester strains.

(A) Initial Screening by Disk Diffusion

Studies using disk diffusion methodology showed that the compound of Formula C, (“TSB007”) (10 mg/ml) had antibacterial activity against a range of organisms, mostly Gram-positive bacteria.

Briefly, blood agar plate (BA) cultures of the 38 organisms listed in Table 5 were prepared over 24 and 48 h for normal and slow-growing organisms, respectively. Pre-dried (30 mins) Mueller Hinton agar plates (MHA; from PathWest Media) were swabbed with 0.5 McFarland suspensions of the BA cultures in saline (0.85% NaCl) as per CLSI guidelines. Anaerobes were instead inoculated onto pre-reduced BA plates, and Streptococcus spp. onto MHA containing 5% sheep blood. Two Whatman 6 mm Antibiotic Assay disks were placed onto each inoculated plate. One disk was impregnated with 20 μl 100% DMSO and the second with 20 μl of 10 mg/ml TSB007 in 100% DMSO. Plates were incubated at 35° C. (with 5% CO₂where mentioned in the Table). Zones were measured after 24 and 48 h.

TSB007 resulted in zones of inhibition of all Gram-positive bacteria tested (Table 5). Zones halved with S. aureus strains, and were no longer seen with S. xylosus, following extended incubation (24 h vs. 48 h), mostly due to the growth of subpopulations of discrete colonies. No zones of inhibition were seen with C. albicans nor with Gram-negative bacteria except M. catarrhalis. Y. enterocolitica had a negligible zone of inhibition after 24 h that was not seen after 48 h.

Photographs were taken after 48 h of all plates where TSB007 produced zones of inhibition. FIGS. 3-5 show a selection of these.

While the above study showed compound TSB007 to be active against Gram-positive bacteria, the zones of inhibition were smaller for the anaerobic Gram-positives. The Gram-negative anaerobe was not inhibited by TSB007. The compound may therefore be less active in an anaerobic environment. Staphylococcus aureus is a facultative anaerobe. The membrane potential is reduced for this organism when grown anaerobically and therefore can affect susceptibility to certain antibiotics.

To confirm the compound was also active against S. aureus in an anaerobic environment, zones of inhibition of S. aureus grown aerobically and anaerobically were compared.

The method described above was used for this investigation except that two MHA plates were prepared for each organism with one examined after aerobic incubation and the other after anaerobic incubation. The organisms tested included S. aureus NCTC 6571, S. aureus NCTC 10442, and S. epidermidis ATCC 12228. The results are shown in Table 6. Zones of inhibition were larger when these organisms were incubated anaerobically compared to aerobically. This was largely due to a lack, when tested anaerobically, of the “creeping” zones (a zone of faint growth within the main zone of inhibition) seen on plates incubated aerobically. No zones of inhibition were seen for DMSO only.

(A) MIC and MBC by Broth Microdilution

The method used was based on CLSI protocols for broth microdilution testing of these species. As recommended by CLSI, inocula were prepared by direct colony suspension, and the media used were cation-adjusted Mueller Hinton broth (CA-MHB) for Staphylococcus spp. and CA-MHB containing 5% lysed horse blood (CA-MHB+LHB) for Streptococcus spp. Briefly, 24 h blood agar plate (BA) cultures of Staphylococcus aureus strains NCTC 6571, ATCC 29213, ATCC 33592 (MRSA) and NCTC 10442 (MRSA), Staphylococcus epidermidis ATCC 29971, Staphylococcus xylosus ATCC 29971, Streptococcus pneumoniae strains ATCC 49619 and ATCC 6305, and Streptococcus pyogenes strains NCTC 8191 and NCTC 8302 were used to prepare 0.5 McFarland suspensions in saline. Two 640 μg/ml working stocks of TSB007 were prepared from a fresh master stock, one in CA-MHB for testing against the Staphylococcus spp. and the other in CA-MHB+LHB for testing against Streptococcus spp. The working stocks were further diluted 1/10 in these broths for adding to microtitre trays to test MICs of 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125 and 0.06 μg/ml.

The wells of two 96-well microtitre trays (one for Staphylococcus spp. and one for Streptococcus spp.) were filled with 100 μl of the appropriate broth, except for the first column. The last column, a sterility control containing no TSB007 or inoculum, was filled with an additional 100 μl broth. Then 100 μl of the 64 μg/ml TSB007 solution was added to the first two columns, the contents of column 2 was mixed, then 100 μl of this was used in two-fold serial dilutions along the rest of the tray except the last two columns. The final 100 μl was discarded. The second last column was used as a growth control so did not have TSB007 added. Finally, to the first 11 wells of one row per strain, 100 μl of a 10⁻²dilution (in the appropriate broth) of the 0.5 McFarland suspension was added. The microtitre trays were then incubated at 35° C. for 24 h before the MICs were read visually. Wells were further subcultured (10 μl drop onto BA then incubated at 35° C. for 24 h) and colonies subsequently counted to determine minimum bactericidal concentrations (MBCs). The concentration of the initial inoculum was confirmed on BA by counting colonies from 2×10 μl spots of 10⁻⁵dilutions of the 0.5 McFarland suspensions. Testing was performed on two separate occasions.

The concentrations of inocula were largely as expected, equating to 1.0×10⁵-6.0×10⁵CFU/ml. Slightly lower concentrations were achieved for S. xylosus ATCC 29971 (5.0-7.5×10⁴CFU/ml) and S. pneumoniae ATCC 49619 (2.5-5.0×10⁴CFU/ml). TSB007 was more active against Staphylococcus spp. (particularly S. aureus) than Streptococcus spp. This was consistent with disc diffusion results above. S. aureus and S. epidermidis had the lowest MICs (4 μg/ml). In general, MBCs were significantly higher than MICs.

The method described above, with some modifications, was used to determine the MIC and MBC for S. aureus NCTC 6571 and E. coli NCTC 10418. Concentrations of the compound were previously too low to determine the MBC of this S. aureus strain.

Modifications were the use of Mueller-Hinton broth (MHB) instead of CA-MHB, the preparation of bacterial inoculum by the growth method instead of direct colony suspension, and the use of more concentrated working stocks and test concentrations of TSB007. Hence, overnight 10 ml MHB cultures prepared from BA cultures were used to make the 0.5 McFarland suspensions in MHB, and doubling dilutions of the working stock at 2048 μg/ml TSB007 were made in MHB across the tray to test MICs of 1024 μg/ml to 0.5 μg/ml. Testing was performed on two separate occasions.

Subsequently, an extended range of concentrations of TSB007 was tested for inhibition of S. aureus NCTC 6571 and E. coli NCTC 10418. The MIC of S. aureus NCTC 6571 was 2 μg/ml, confirming the previous MIC result for this strain, and the MBC was 128-256 μg/ml. The MIC and MBC for E. coli NCTC 10418 were 512 μg/ml and >1024 μg/ml, respectively.

An overnight 10 ml MHB culture of S. aureus NCTC 6571 was diluted to 0.5 McFarland in MHB then 1 ml was used to inoculate each of four 100 ml flasks prepared as follows:

• • 1) 9.0 ml MHB (control)
  • 2) 9.99 ml MHB plus 9.7 μl of TSB007 working stock at 2048 μg/ml (1.8 μg/ml)
  • 3) 9.98 ml MHB plus 19.5 μl of TSB007 working stock at 2048 μg/ml (3.6 μg/ml)
  • 4) 6.74 ml MHB plus 256 μl of a TSB007 master stock (320 μg/ml). This equated to 3.2% DMSO which did not have any effect on the viability of S. aureus NCTC 6571 (data not shown).

The flasks were incubated at 37° C. for 10 min before inoculation. Samples (0.1 ml) were taken immediately after inoculation (time 0) and then at 15, 30, 60, 120 and 240 min. The samples were immediately diluted 10⁻¹in 0.01 M phosphate buffered saline at pH 7.0 (PBS). Further 10-fold dilutions in PBS were made down to 10⁻⁴until the 60 min time point, and then down to 10⁻⁶for the remaining time points. Viable counts were estimated using 3×20 μl drops from each dilution on MHA which was then incubated at 37° C. overnight before counting. These were normalised against the initial counts from each flask. Testing was performed on three separated occasions.

The viability of S. aureus NCTC 6571 cultures containing TSB007 at 1.8 μg/ml (NB. The MIC was 2 μg/ml), 3.6 μg/ml (1.8×MIC) and at 320 μg/ml (1.25×MBC) was tested. The initial concentration of microorganism in each flask was 0.5-1.0×10⁷CFU/ml. At both 1.8 μg/ml and 3.6 μg/ml, TSB007 was inhibitory for the 4 h of the experiment (FIG. 6). At 24 h, the cells with TSB007 at 1.8 μg/ml had grown to counts of 10⁸CFU/ml, and those with 3.6 μg/ml to 10³-10⁸CFU/ml. The cells with 320 μg/ml at the first time point of 15 min were below the limit of detection with a >99.9% kill.

Various analogues of TSB007 were synthesised and tested in DMSO in disk diffusion assays against S. aureus NCTC 6571 using the protocol of Example 3. A number of compounds were also tested against E. coli NCTC 10418.

Compounds TSB065 and TCB001 were successful at inhibiting growth of S. aureus NCTC 6571, with TSB065 giving a zone of inhibition of 14 mm and TCB001 giving a zone of inhibition of 11 mm.

Several analogues were also tested against a range of microorganisms as shown in Table 6.

Compounds DSB001, DSD002, DSB004, TSB008 and QSB002 showed no zones of inhibition with S aureus NCTC6571 when applied at 10 mg/ml.